The in vitro culture of embryonic chicken heart cells.

IN the early 1900’s, Burrows [l ] and Carrel [2] reported the serial cultivation of cells derived from an adult chicken heart. Since that time, numerous laboratories have experienced difficulties in long-term avian cell culture. After many unsuccessful attempts, with several modifications in technic, embryonic chick heart cells were established and maintained in this laboratory as monolayer cultures. Two such strains were originated independently; they are designated as CH,, and CH,, respectively, and are in the 41st and 28th subculture at the time of this writing.